We introduce an innovative fabrication of the polymer scaffolds for tissue culture by utilizing the evaporation induced self-assembled micropatterns of poly styrene-block-poly(acrylic acid) (PS-b-PAA) diblock copolymer micelles. The microstructures were used as templates for micromolding a silicon elastomer, poly(dimethylsiloxane) (PDMS), into tissue scaffolds and microwells for cell patterning purpose. Cultivation of human epithelial cells (Calu-3 cell line) on the PDMS scaffolds demonstrates potential applications in tissue engineering and cell-based biosensors. The reported method is rapid, simple, economical, and versatile comparing with the existing microfabrication techniques. (c) 2006 Elsevier Ltd. All rights reserved.