In this paper an immobilization of invertase on thermosensitive copolymers of N-isopropylacrylamide and 2-hydroxyethyl methacrylate or glycidyl methacrylate modified by aminolysis is evaluated. The method is based on the swelling properties of stimuli-sensitive polymers, which work like a pump that sucks up the enzyme on cooling and then on subsequent crosslinking of the enzyme. The attention was focused on the properties of the carrier-enzyme systems, particularly on the effect of crosslinking on their stability. Activity of TG8-NH2 carrier was very low and independent on concentration of glutaraldehyde used, but carriers TH8 and TH8-NH2 were more active, especially when 1.0 and 2.5 vol.% of glutaraldehyde were used. It was also observed, that preparations crosslinked by glutaraldehyde were more stable than preparations without crosslinking agent. (c) 2005 Elsevier Inc. All rights reserved.