화학공학소재연구정보센터
Chemical Engineering Science, Vol.52, No.2, 171-176, 1997
Growth-Kinetics of L-Aminoacylase-Producing Pseudomonas Sp Ba2
The growth of an L-aminoacylase-producing bacterium, Pseudomonas sp. BA2, was modelled by using a model based on a modification of the Monod equation. The modelling was carried out by direct simultaneous integration of the differential equations for cell growth and substrate consumption. The values of the kinetic parameters were evaluated by numerical fitting of the experimental results obtained in different fermentations at various substrate concentrations, under the optimal experimental conditions. The variation of mu(max) with the operational temperature was found to follow the Arrhenius relationship. The influence of the pH on the maximum growth rate could be predicted by using one of the equations used in enzyme kinetics. The good agreement between the experimental and theoretical data (mean errors in the range 2-7.84%) shows that the proposed unstructured model is adequate to describe the growth of the bacterial strain.