Studies on cultivation and biological activities of Pleurotus nebrodensis inzenga

Du Bok Choi; Hyung Gun Nam; Wol-Suk Cha

Korean Journal of Chemical Engineering, Vol.23, No.2, 241-246, March, 2006

DOI10.1007/BF02705723 Export Citation

Studies on cultivation and biological activities of Pleurotus nebrodensis inzenga

Du Bok Choi, Hyung Gun Nam, and Wol-Suk Cha^†

Dept. of Chemical Engineering, Chosun University, Gwang-ju 501-759, Korea

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Environmental factors affecting mycelial growth and exo-polysaccharide production from Pleurotus nebroden-sis Inzenga (PN) and biological activities of PN extracts in vitro were studied. The culture conditions for effective my-celial growth and exo-polysaccharide production were found to be 25oC, 5% of inoculum size, and an initial pH from6.5 to 7.0. When 5% of glucose was used, the maximum mycelial growth and exo-polysaccharide concentrations were8.3 and 3.07g/L, respectively. Among the various nitrogen sources, the mycelial growth and exo-polysaccharide pro-duction were very strong when polypeptone was used. In the case of the minerals sources, K2HPO4 and MgSO4·7H2Owere found to best support for mycelial growth and exo-polysaccharide production. Under optimal conditions and meth-ods, the maximum mycelial growth and exo-polysaccharide production were obtained after 10 days of culture, 12.84and 4.85g/L, respectively, in a jar fermentor. The effects of the PN extracts on the viability of three human cancer celllines and antioxidant activity were investigated in vitro. When cancer cells of the lung (A549), cervical region (HeLa)and colon (KM12C) were incubated at 6 mg/mL of the PN ethanol extracts, the viabilities of the HeLa and KM12Ccells were slightly decreased. However, the growth of the A549 cells was remarkably inhibited when the PN ethanolextract was over 4mg/mL. The antioxidant activity showed 46.2% at 40μL, which was about 3.2 fold higher thanthat of the PN methanol extract.

Keywords:Pleurotus nebrodensis Inzenga;Exo-polysaccharide;Anti-tumor;Antioxidant

[References]

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Bobek P, Galbavy S, J. Biomed. Sci., 58, 164 (2001)
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Halliwell B, Gutteridge JMC, Biochem. J., 219, 1 (1984)
Hong IP, Lee MW, Kor. J. Mycol., 18, 42 (1990)
Hong JS, Choi YH, Yun SE, Kor. J. Mycol., 14, 121 (1986)
Huang N, Colored illustrations of macrofungi mushrooms of China, China Agricultural Press, Beijing, pp. 23-29 (1998)
Jung IC, Kim SH, Kwon YI, Kim SY, Lee JS, Park S, Park KS, Lee JS, Kor. J. Mycol., 25, 133 (1997)
Kim HH, Na JG, Chang YK, Chun GT, Lee SJ, Jeong YH, J. Microbiol. Biotechnol., 14(5), 944 (2004)
Kim HK, Park JS, Cha DY, Kim YS, Moon BJ, Kor. J. Mycol., 22, 145 (1994)
Noda S, “Antibiotic fungicide extracted from Basidiomycetes culture (e.g. Shitake, Flammulina velutips, Polyporus, Pleurotus, etc.),” J P Patent 8445268 (1998)
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Oguri S, Ando A, Nagata Y, J. Bacteriol., 178, 5692 (1996)
Ohga S, J. Wood Sci., 45, 431 (1999)
Opletal L, Jahodar L, Chabot V, Zdansky P, Lukes J, Bratova M, Solichova D, Blunden G, Dacke CG, Patel A, Br. Biomed. Sci., 54, 240 (1997)
Park KS, Park S, Jung IC, Ha HC, Kim SH, Lee JS, Kr. J. Myco, 22, 184 (1994)
Paulik S, Svreck S, Mojzisova J, Durove A, Benishek Z, Huska M, Zentrabl Vecterinaermed Reihe B., 43, 129 (1996)
Yang FC, Liau CB, Process Biochem., 33(5), 547 (1998)
Yang FC, Ke YF, Kuo SS, Enzyme Microb. Technol., 27(3-5), 295 (2000)
Zadrazil F, Mushroom Sci., 9, 621 (1974)

[Referenced By]

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[2] Bobek P, Galbavy S, J. Biomed. Sci., 58, 164 (2001)

[3] Cha WS, Choi DB, Kang SH, Biotech. Biopro. Bioeng., 9, 369 (2004)

[4] Chi JH, Ha TM, Kim YH, Rho YD, Korean J. Mycol., 24, 214 (1996)

[5] Choi DB, Kang SH, Song YH, Kwun KH, Jeong KJ, Cha WS, J. Microbiol. Biotechnol., 15, 368 (2005)

[6] Hashimoto K, Takahashi Z, Mush. Sci., 9, 585 (1974)

[7] Halliwell B, Gutteridge JMC, Biochem. J., 219, 1 (1984)

[8] Hong IP, Lee MW, Kor. J. Mycol., 18, 42 (1990)

[9] Hong JS, Choi YH, Yun SE, Kor. J. Mycol., 14, 121 (1986)

[10] Huang N, Colored illustrations of macrofungi mushrooms of China, China Agricultural Press, Beijing, pp. 23-29 (1998)

[11] Jung IC, Kim SH, Kwon YI, Kim SY, Lee JS, Park S, Park KS, Lee JS, Kor. J. Mycol., 25, 133 (1997)

[12] Kim HH, Na JG, Chang YK, Chun GT, Lee SJ, Jeong YH, J. Microbiol. Biotechnol., 14(5), 944 (2004)

[13] Kim HK, Park JS, Cha DY, Kim YS, Moon BJ, Kor. J. Mycol., 22, 145 (1994)

[14] Noda S, “Antibiotic fungicide extracted from Basidiomycetes culture (e.g. Shitake, Flammulina velutips, Polyporus, Pleurotus, etc.),” J P Patent 8445268 (1998)

[15] Noda S, “Preparation of an antiviral agent by deep culture Basidiomycetes,” J P Patent 83162084 (1998)

[16] Noda S, “A preparation for kindey treatment possessing antiflammatory activity, obtained from Basidiomycetes, e.g. Lentinus, Pleurotus, Flammulina, and Tricholoma,” J P Patent 8511888 (1998)

[17] Oguri S, Ando A, Nagata Y, J. Bacteriol., 178, 5692 (1996)

[18] Ohga S, J. Wood Sci., 45, 431 (1999)

[19] Opletal L, Jahodar L, Chabot V, Zdansky P, Lukes J, Bratova M, Solichova D, Blunden G, Dacke CG, Patel A, Br. Biomed. Sci., 54, 240 (1997)

[20] Park KS, Park S, Jung IC, Ha HC, Kim SH, Lee JS, Kr. J. Myco, 22, 184 (1994)

[21] Paulik S, Svreck S, Mojzisova J, Durove A, Benishek Z, Huska M, Zentrabl Vecterinaermed Reihe B., 43, 129 (1996)

[22] Yang FC, Liau CB, Process Biochem., 33(5), 547 (1998)

[23] Yang FC, Ke YF, Kuo SS, Enzyme Microb. Technol., 27(3-5), 295 (2000)

[24] Zadrazil F, Mushroom Sci., 9, 621 (1974)