Ethosomes의 포집효율과 입자크기에 영향을 주는 인자에 관한 연구

진병석; 이상묵; 이광희; Byung Suk Jin; Sang Mook Lee; Kwang Hee Lee

Journal of the Korean Industrial and Engineering Chemistry, Vol.17, No.2, 138-143, April, 2006

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Ethosomes의 포집효율과 입자크기에 영향을 주는 인자에 관한 연구

A Study on the Factors Affecting Entrapment Efficiency and Particle Size of Ethosomes

진병석 ^†, 이상묵 , 이광희 ¹

동덕여자대학교 응용화학과
¹인하대학교 나노시스템 공학부

Byung Suk Jin^†, Sang Mook Lee , and Kwang Hee Lee¹

Department of Applied Chemistry, Dongduk Women’s University, Seoul 136-714, Korea
¹Nano System Engineering, Inha University, Inchon 402-751, Korea

E-mail:

초록

Ethosome은 에탄올에 용해된 레시틴을 친수성 용액으로 수화시켜 만들어지는 액정형 베시클이다. Ethosome을 약물전달체로 개발하기 위해서는 베시클의 높은 포집효율과 작은 입자크기가 필수적이기 때문에 ethosome의 포집효율과 입자크기에 영향을 주는 인자들에 대한 연구를 시도하였다. Calcein을 친수성 지표물질로 사용하여 ethosome을 만들고, 구성 성분비와 제조조건에 따른 ethosome의 특성의 변화를 관찰하였다. 에탄올과 calcein 용액의 첨가량 레시틴 중 포스파티딜콜린의 함량, 제조온도, 교반속도 및 PBS 첨가방법 등이 ethosome의 특성에 상당히 큰 영향을 미치는 것을 확인하였다. 초음파 처리를 한 경우에는 ethosome의 포집효율이 감소하는 결과가 나타났는데 이러한 결과는 강한 초음파 진동에 의해 베시클에 포집되었던 성분이 방출되었기 때문이다.

Ethosome is a liquid crystalline vesicle prepared by hydration of ethanol-dissolved lecithin with a solution containing hydrophilic components. Investigation of factors affecting the entrapment efficiency and particle size of ethosomes was carried out, because the high entrapment efficiency and small particle size are prerequisite in developing ethosomes as a drug delivery system. The variations of properties of ethosomes with constituent composition and preparation method were examined using a calcein as a hydrophilic marker. It was observed that the amount of ethanol and calcein solution, phosphatidyl choline content in lecithin, preparation temperature, stirring rate, and PBS addition method had a considerable effect on the properties of ethosome. Sonication treatment resulted in the reduction of entrapment efficiency of ethosome, which was due to the release of entrapped components in the vesicles by strong sonication vibration.

Keywords:ethosome;hydrated liquid crystalline vesicle;entrapment efficiency;particle size;lecithin

[References]

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Knepp VM, Hinz RS, Szoka FC, Guy RH, J. Control. Release, 5, 211 (1988)
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Trotta M, Peira E, Carlotti ME, Gallarate M, Int. J. Pharm., 270, 119 (2004)
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Touitou E, Meidan VM, Horwitz E, J. Control. Release, 56, 7 (1998)
Perrett S, Golding M, Williams WP, J. Pharm. Pharmacol., 43, 154 (1991)
Kirjavaiene M, Urtti A, Valjakka-Koskela R, Kiesvaara J. Monkkonen J, Eur. J. Pharm. Sci., 7, 279 (1999)
Jin BS, Han YM, Lee KH, Choi MJ, J. Korean Ind. Eng. Chem., 15(2), 205 (2004)

[Referenced By]

[Related Articles]

[1] Egbaria K, Weiner N, Adv. Drug Deliv. Rev., 5, 287 (1990)

[2] Knepp VM, Hinz RS, Szoka FC, Guy RH, J. Control. Release, 5, 211 (1988)

[3] Knepp VM, Szoka FC, Guy RH, J. Control. Release, 12, 25 (1990)

[4] Planas ME, Gonzalez P, Rodriquez L, Sanchez S, Cevc G, Anesth. Analg., 75, 615 (1992)

[5] Cevc G, Blume G, Biochim. Biophys. Acta., 1104, 226 (1992)

[6] Cevc G, Schatzlein A, Blume G, J. Control. Release, 36, 3 (1995)

[7] Cevc G, Blume G, Schatzlein A, Gebauer D, Paul A, Adv. Drug Deliv. Rev., 18, 349 (1996)

[8] Cevc G, Blume G, Schatzlein A, J. Control. Release, 45, 211 (1997)

[9] Paul A, Cevc G, Bachhawat BK, Eur. J. Immunol., 25, 3521 (1995)

[10] Trotta M, Peira E, Debernardi F, Gallarate M, Int. J. Pharm., 241, 319 (2002)

[11] Trotta M, Peira E, Carlotti ME, Gallarate M, Int. J. Pharm., 270, 119 (2004)

[12] Touitou E, Dayan N, Bergelson L, Godin B, Eliaz M, J. Control. Release, 65, 403 (2000)

[13] Touitou E, Godin B, Weiss C, Drug Dev. Res., 50, 406 (2000)

[14] Touitou E, Meidan VM, Horwitz E, J. Control. Release, 56, 7 (1998)

[15] Perrett S, Golding M, Williams WP, J. Pharm. Pharmacol., 43, 154 (1991)

[16] Kirjavaiene M, Urtti A, Valjakka-Koskela R, Kiesvaara J. Monkkonen J, Eur. J. Pharm. Sci., 7, 279 (1999)

[17] Jin BS, Han YM, Lee KH, Choi MJ, J. Korean Ind. Eng. Chem., 15(2), 205 (2004)