It was indicated that a charged mosaic membrane could preferentially transfer electrolytes more readily than solvent(negative reflection coefficient). In this study, material transport across the charged mosaic membrane was investigated under different experiment conditions. KCl was added in one phase and sucrose was added in other one across charged mosaic membrane (KCl/ Sucrose system). The identical and equivalent solute concentrations were kept in both phases to cancel each other's osmotic flow. The volume fluxes and the solute fluxes on the system were measured and the reflection coefficients which expressed the separation factor between solvent and solute were estimated. The values were compared with KCl/water system. As a result, the volume fluxes of KCl/sucrose system were higher and the solute fluxes were lower than that of KCl/water. In addition, the reflection coefficients were almost the same values in both systems. As a whole, the KCl/sucrose system indicated lower desalination than the KCl/water system.