Adsorption and desorption behaviors of S-adenosyl-L-methionine in a fixed-bed ion-exchange column

Zhinan Xu; Yong Chen; Wenhe Shen; Peilin Cen

Korean Journal of Chemical Engineering, Vol.23, No.1, 108-112, January, 2006

DOI10.1007/BF02705700 Export Citation

Adsorption and desorption behaviors of S-adenosyl-L-methionine in a fixed-bed ion-exchange column

Zhinan Xu^†, Yong Chen¹, Wenhe Shen, and Peilin Cen

Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China
¹Pharmaceutical Informatics Institute, Department of Chinese Medicine Science and Engineering, Zhejiang University, Hangzhou 310027, China

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S-adenosyl-L-methionine (SAM) is one of most versatile molecules in nature and has wide medical applications. The ion-exchange separation process of SAM of the extract of yeast cells has many advantages over selective precipitation by picrolonic acid. Experiments of the dynamic column process of SAM on JK110 resin were carried out in a fixed-bed ion-exchange column. The effects of different operation parameters on the adsorption and desorption behaviors of SAM were investigated. The results show that the ion-exchange adsorption of SAM is successfully implemented at 2BV/h, 10 g/L, pH 5.0; the adsorbed SAM in the ion-exchanged bed is efficiently desorbed by 0.2 N H2SO4 solution at the flow rate of 2BV/h. According to material balance, the recovery yield of SAM for this ion-exchange process is 90.1%. Finally, this ion-exchange process was successfully scaled up to separate SAM at high yield and purity.

Keywords:S-Adenosyl-L-Methionine;JK110 Resin;Fixed-Bed Ion-Exchange;Separation

[References]

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Morana A, Paola S, Giovanni C, Monica L, Maria C, Mario DR, Biochimica et Biophysica Acta, 1573, 105 (2002)
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[Referenced By]

[1] Charles SL, J. Hepatol., 30, 1155 (1999)

[2] Chen Y, Xu Z, Shen W, Lin J, Cen P, Korean J. Chem. Eng., 22(1), 121 (2005)

[3] Farooqui J, Kim S, Paik WK, Electroanalysis, 4, 261 (1983)

[4] Alberto F, US patent, 4028183 (1977)

[5] Ftiedel HA, Goa KL, Benfield P, Drugs, 38, 389 (1989)

[6] Gaetano B, Rita C, Maria CF, Gabriella DA, Mario DR, Roberto S, Silvama W, Vincenzo Z, Biochimic at Biophysica Acta, 991, 324 (1989)

[7] Gennari and Fedrico, US patent, 4990606 (1991)

[8] Lin JP, Tian J, You JF, Jin ZH, Xu ZN, Cen PL, Biochem. Eng. J., 21, 19 (2004)

[9] Liu H, "Techniques and mechanism of the co-production of S-adenosyl-L-methionine and glutathione using the yeast" (Doctor Dissertation), Hangzhou: College of materials science and chemical engineering, Zhejiang University (2002)

[10] Liu H, Lin JP, Can PL, Pan YJ, Process Biochem., 39, 1993 (2004)

[11] Morana A, Paola S, Giovanni C, Monica L, Maria C, Mario DR, Biochimica et Biophysica Acta, 1573, 105 (2002)

[12] Morana A, Lernia ID, Carteni M, Rose RD, Rosa MD, Int. J. Pharm., 194, 61 (2000)

[13] Pascale RM, Marras V, Simile MM, Daino L, Pinna G, Bennati S, Carta M, Seddaiu MA, Massarelli G, Feo F, Cancer Res., 52, 4979 (1992)

[14] Shelly CL, Int. J. Biochem. Cell Biol., 32, 391 (2000)

[15] Vettere G, Am. J. Med., 83, 78 (1987)