A new class of protein-resistant film based on N-substituted glycine derivatives is described. Pulsed plasma deposited poly(N-acryloylsarcosine methyl ester) coatings are shown to be resistant toward the adsorption of fibrinogen and lysozyme. Deposition and UV irradiation of the polymer through a masked grid are found to be effective ways for generating negative and positive image protein arrays, respectively, onto a range of different substrate materials.