A method to create assembly of polydiacetylene vesicles on solid Substrates in a novel fashion is described. The formation of the assembly is based on electrostatic layer-by-layer deposition using negatively charged 10, 12-pentacosadiynoic acid (PCDA) vesicles and polyelectrolyte polyethylenimine, or positively charged PCDA-2'-aminoethylamide (PCDANH(2)) vesicles. This is an efficient method for preparing the chromatic sensor films of polydiacetylene vesicles after ultraviolet light irradiation. The strategy would be useful in the development of polydiacetylene-based chemosensors and biosensors. (c) 2005 Elsevier Inc. All rights reserved.