Transition of mechanical property of porous alginate scaffold with cells during culture period

Sakai S; Masuhara H; Yamada Y; Ono T; Ijima H; Kawakami K

Journal of Bioscience and Bioengineering, Vol.100, No.1, 127-129, 2005

Sakai S, Masuhara H, Yamada Y, Ono T, Ijima H, Kawakami K

The rupture forces of porous alginate scaffolds seeded with hepatocytes or fibroblast-like cells increased during 3 d of culture and then decreased. The phenomenon was independent of the number of viable cells within the scaffolds, but dependent on protein adsorption to the alginate as well as a reduction in the degree of crosslinks of the calcium-alginate gel.

Keywords:alginate;scaffold;cell culture;sponge;tissue engineering