Energy dissipation chemical force microscopy has been used to analyze the dissipative properties of chemically similar regions of hydroxyl-and carboxyl-terminated SAMS on gold with a hydroxyl-terminated tip. Energy dissipation imaging quantitatively isolates dissipative interfacial interactions from topography, producing a significantly more informative image than phase imaging. Also, energy dissipation force curves probed the theological properties of the tip-sample interaction. Viscosity of the confined water increased slightly over that of the bulk, and SAM deformation was found to have a longer retardation time than restructuring of interfacial ions and solvent during tip-sample contact.