Degradation of Styrene by a New Isolate Pseudomonas putida SN1

Mi So Park; Ju Hee Han; Seung Shick Yoo; Eun Yeol Lee; Sun Gu Lee; Sunghoon Park

Korean Journal of Chemical Engineering, Vol.22, No.3, 418-424, May, 2005

DOI10.1007/BF02719421 Export Citation

Degradation of Styrene by a New Isolate Pseudomonas putida SN1

Mi So Park, Ju Hee Han, Seung Shick Yoo, Eun Yeol Lee¹, Sun Gu Lee , and Sunghoon Park^†

Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Korea
¹Department of Food Science and Technology, Kyungsung University, Busan 608-736, Korea

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Twelve styrene-utilizing bacteria were isolated from a biofilter used for treating gaseous styrene. A gramnegative strain had a high styrene-degrading activity and was identified as Pseudomonas putida SN1 by 16S rDNA analysis. The styrene degradation in SN1 was regarded to start with a monooxygenase enzyme which converted styrene to styrene oxide, a potentially important chiral building block in organic synthesis. SN1 could grow on styrene and styrene oxide, but not on benzene and toluene. The styrene degradation activity in SN1 was induced when incubated with styrene, and the induction was not inhibited by the presence of readily usable carbon sources such as glucose and citrate. The optimal activity was shown at pH 7.0 and 30 ℃ and estimated as 170 unit/g cell.

Keywords:Styrene;Styrene Degrading Microorganisms;Styrene Monooxygenase (SMO);Pseudomonas putida SN1;Induction of SMO Activity

[References]

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Besse P, Veschambre H, Tetrahedron, 50, 8885 (1994)
Collins AN, Sheldrake GN, Crosby J, Chirality in Industry, John Wiley & Sons, New York (1992)
Cox HHJ, Faber BW, vanHeiningen WNM, Radhoe H, Doddema HJ, Harder W, Appl. Environ. Microbiol., 62, 1471 (1996)
Gennaro PD, Colmegna A, Galli E, Sello G, Pelizzoni F, Bestetti G, Appl. Environ. Microbiol., 65, 2794 (1999)
Grogan CR, Willertts A, Enzymatic, 3, 253 (1997)
Han JH, Production of Enantiopure (S)-Styrene Oxide Using a Mutant of Pseudomonas putida Lacking Styrene Oxide Isomerase, Thesis for masters degree, Pusan National University (2004)
Hartmans S, Smits JP, vanderWerf MJ, Volkering F, deBont JAM, Appl. Environ. Microbiol., 55(11), 2850 (1989)
Hartmans S, vanderWerf MJ, deBont JAM, Appl. Environ. Microbiol., 56(5), 1347 (1990)
Hasegawa J, Ohashi T, Chem. Today, 44 (1996)
Hollmann F, Lin PC, Witholt B, Schmid A, J. Am. Chem. Soc., 125(27), 8209 (2003)
Itoh N, Yoshida K, Okada K, Biosci. Biotechnol. Biochem., 60(11), 1826 (1996)
Kang JM, Biological Degradation of Ethylene Chlorides using Methylosinus trichosporium OB3b, Thesis for master degree, Pusan National University (1998)
Kim JR, Biological Production of Hydrogen from Carbno Monoxide and Water by the Novel Chemoheterotrophic Bacterium Citrobacter sp. Y19, Thesis for master degree, Pusan National University (2000)
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Klasson KT, Lundback KMO, Clausen EC, Gaddy JL, J. Biotechnol., 29, 177 (1993)
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Panke S, deLorenzo V, Kaiser A, Witholt B, Wubbolts MG, Appl. Environ. Microbiol., 65, 5619 (1999)
Panke S, Witholt B, Schmid A, Wubbolts M, Appl. Environ. Microbiol., 64(6), 2032 (1998)
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Santos PM, Blatny JM, deBartolo I, Valla S, Zennaro E, Appl. Environ. Microbiol., 66(4), 1305 (2000)
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Wubbolts MG, Reuvekamp P, Witholt B, Enzyme Microb. Technol., 16(7), 608 (1994)
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[2] Besse P, Veschambre H, Tetrahedron, 50, 8885 (1994)

[3] Collins AN, Sheldrake GN, Crosby J, Chirality in Industry, John Wiley & Sons, New York (1992)

[4] Cox HHJ, Faber BW, vanHeiningen WNM, Radhoe H, Doddema HJ, Harder W, Appl. Environ. Microbiol., 62, 1471 (1996)

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[8] Hartmans S, Smits JP, vanderWerf MJ, Volkering F, deBont JAM, Appl. Environ. Microbiol., 55(11), 2850 (1989)

[9] Hartmans S, vanderWerf MJ, deBont JAM, Appl. Environ. Microbiol., 56(5), 1347 (1990)

[10] Hasegawa J, Ohashi T, Chem. Today, 44 (1996)

[11] Hollmann F, Lin PC, Witholt B, Schmid A, J. Am. Chem. Soc., 125(27), 8209 (2003)

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[13] Kang JM, Biological Degradation of Ethylene Chlorides using Methylosinus trichosporium OB3b, Thesis for master degree, Pusan National University (1998)

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[15] Kim SB, Yoon JH, Kim H, Lee ST, Park YH, Goodfellow M, Int. J. Syst. Bacteriol., 45, 351 (1995)

[16] Klasson KT, Lundback KMO, Clausen EC, Gaddy JL, J. Biotechnol., 29, 177 (1993)

[17] Marconi AM, Beltrametti F, Bestetti G, Solinas F, Ruzzi M, Galli E, Zennaro E, Appl. Environ. Microbiol., 62, 121 (1996)

[18] Martinez-Blanco H, Reglero A, Rodriquez-Aparacio LB, Luengo JM, J. Biol. Chem., 265, 7084 (1990)

[19] Nothe C, Hartmans S, Biocatalysis, 10, 219 (1994)

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[21] O'Connor KE, Buckley CM, Hartmans S, Dobson DW, Appl. Environ. Microbiol., 61, 544 (1995)

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[26] Santos PM, Blatny JM, deBartolo I, Valla S, Zennaro E, Appl. Environ. Microbiol., 66(4), 1305 (2000)

[27] Sheldon RA, Chemtech. September, 566, 576 (1991)

[28] Shiray K, Hisatsuka K, Agric. Biol. Chem., 43, 1595 (1979)

[29] Shiray K, Hisatsuka K, Agric. Biol. Chem., 43, 1399 (1979)

[30] Velasco A, Alonso S, Garcia JL, Perera J, Diaz E, J. Bacteriol., 180(5), 1063 (1998)

[31] Warhurst AM, Fewson CA, J. Appl. Bacteriol., 77, 597 (1994)

[32] Wubbolts MG, Reuvekamp P, Witholt B, Enzyme Microb. Technol., 16(7), 608 (1994)

[33] Yabuuchi E, Kosako Y, Oyaizu H, Yano I, Hotta H, Hashimoto Y, Ezaki T, Arakawa M, Microbiol. Immunol., 36, 1251 (1992)

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[35] Yeom SH, Yoo YJ, Korean J. Chem. Eng., 19(5), 780 (2002)