In this work, chiral activated membranes have been tested for the enantio-separation of the racemic drug propranolol. Polysulfone (PS)-based membranes have been prepared for this purpose, using N-hexadecyl-L-hydroxyproline (HHP) and L-di-n-dodecyltartrate (L-DDT) as chiral carriers, respectively. Kinetic experiments have been carried out using a membrane module with two rectangular channels separated by the membrane, which allows for a well-defined hydrodynamics of the feed and stripping phases.