We have found an interesting immobilization technique of liposomes on electron-beam exposed resist surfaces. The immobilized liposomes have been visualized by the atomic force microscope and have shown well-organized three-dimensional physical structures, in which the liposomes maintain their shapes and sizes similar to those of the original design in prepared solution. The immobilization is based on a strong static charge interaction between the resist surface and the liposomes. Further experiments show that very strong charge in the surfaces produces the firm immobilization of the liposome. We believe these findings can be related to various liposome applications such as drug delivery system, electrochemical or biosensors, and nanoscale membrane function studies.