Journal of Bioscience and Bioengineering, Vol.98, No.2, 136-139, 2004
In planta transformation of kenaf plants (Hibiscus cannabinus var. aokawa No. 3) by Agrobacterium tumefaciens
Kenaf was transformed by inoculation of Agrobacterium tumefaciens onto the meristems of young plants in pots. The transformation was demonstrated by three lines of evidence: a phenotypic inheritance from T-0 to T-1 plants, detection of the transgene in both T-0 and T-1 plants, and rescue of plasmids composed of T-DNA of the binary vector and flanking plant genomic DNA from T-1 plants.
Keywords:Agrobacterium tumefaciens;in planta transformation;kenaf (Hibiscus cannabinus);nested PCR;rescue of T-DNA insertion locus