Biotechnology Letters, Vol.26, No.16, 1273-1276, 2004
Coupled-enzyme system for the determination of lipase activity
A new method for lipase activity determination is described, in which liberation of fatty acids by lipase-catalyzed hydrolysis is coupled to lipoxygenation. The second reaction forms hydroperoxy-fatty acids containing a conjugated double bond that are detected at 234 nm. The method is sensitive, cheap and easy to use when compared to a titration method.