Fermentation experiments using Aspergillus niger result in a very viscous broth due to the growth of filamentous microorganism. For viscous fermentation processes, it is difficult to estimate with confidence the volumetric oxygen mass transfer coefficient (K(L)alpha), which can be used for scale-up or design of bioreactors. In the present study, four methods based on dynamic and stationary approaches were used to measure K(L)alpha throughout the fermentation. Data reconciliation was used to obtain a more reliable and consistent K(L)alpha. The K(L)alpha value obtained by a data reconciliation technique was found to be more reliable since it takes into consideration both the reliability of all measured variables and the accuracy of all mass balance equations.