Expression of ornithine decarboxylase of Coccidioides immitis in three Escherichia coli strains carrying the lambda DE3 lysogen and an E-coli EWH319 strain odc(-) null mutant

Pantoja-Hernandez MA; Munoz-Sanchez CI; Guevara-Gonzalez RG; Botello-Alvarez E; Gonzalez-Chavira MM; Torres-Pacheco I; Guevara-Olvera L

Biotechnology Letters, Vol.26, No.1, 75-78, 2004

DOI10.1023/B:BILE.0000009465.08712.a6 Export Citation

Expression of ornithine decarboxylase of Coccidioides immitis in three Escherichia coli strains carrying the lambda DE3 lysogen and an E-coli EWH319 strain odc(-) null mutant

Pantoja-Hernandez MA, Munoz-Sanchez CI, Guevara-Gonzalez RG, Botello-Alvarez E, Gonzalez-Chavira MM, Torres-Pacheco I, Guevara-Olvera L

Ornithine decarboxylase from respiratory fungal pathogen, Coccidioides immitis, cloned in the pETCiODC plasmid under control of T7lac promoter, was produced in E. coli BL21(DE3), BL21( DE3) pLysS, BLR(DE3) and EWH319 transformant strains. E. coli BL21( DE3) pLysS-pETCiODC expressed the highest specific activity of ODC, suggesting that this strain could be successfully used for protein structure and drug testing studies.

Keywords:Coccidioides immitis;Escherichia coli;gene function;lambda DE3 lysogen;protein expression