Quantitative Characterization of the Effects of Concentration and Structural Stability on T4 Lysozyme Adsorption

Man Jin Mo; Woo-Kul Lee

Journal of Industrial and Engineering Chemistry, Vol.10, No.5, 752-758, September, 2004

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Quantitative Characterization of the Effects of Concentration and Structural Stability on T4 Lysozyme Adsorption

Man Jin Mo, and Woo-Kul Lee^†

Laboratory of Biomaterials and Biointerface Research, Department of Chemical Engineering, Dankook University, Seoul 140-714, Korea

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In this study, a simulation model for the adsorption of bacteriophage T4 lysozyme onto hydrophilic silica surfaces has been investigated. The concentration-dependent adsorption mechanism has been analyzed with respect to an adsorption model that allows two different adsorbed states. By utilizing a pair of stability mutants of T4 lysozyme, the adsorption behavior was characterized in terms of two adsorption parameters, α and β. The adsorption parameters in the model were represented as a function of the adsorption time. The simulation results demonstrate that the estimated model parameters predict the adsorption kinetic data appropriately at all of the concentrations used.

Keywords:protein adsorption;simulation;ellipsometry;T4 lysozyme

[References]

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Cho D, J. Ind. Eng. Chem., 6(5), 325 (2000)
Lee WK, McGuire J, Bothwell MK, J. Colloid Interface Sci., 252(2), 473 (2002)
Langmuir I, J. Am. Chem. Soc., 40, 1361 (1918)
Choi HJ, Lee H, Kim KR, Ahn DH, J. Ind. Eng. Chem., 8(6), 586 (2002)
Aizenbud B, Volterra V, Priel Z, J. Colloid Interface Sci., 103, 133 (1985)
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Lundstrom I, Prog. Colloid Polym. Sci., 70, 76 (1985)
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Djardin P, Cottin I, Colloids Surf. B: Biointerfaces, 4, 111 (1995)
Matthews BW, Dahlquist FW, Maynard AY, J. Mol. Biol., 78, 575 (1973)
Weaver LH, Mathews BW, J. Mol. Biol., 193, 189 (1987)
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Mcguire J, Wahlgren MC, Arnebrant T, J. Colloid Interface Sci., 170(1), 182 (1995)
Mcguire J, Wahlgren MC, Arnebrant T, J. Colloid Interface Sci., 170(1), 193 (1995)
Cuypers PA, Corsel JW, Janssen MP, Kop JMM, Hermens WT, Hemker HC, J. Biol. Chem., 258, 2426 (1983)
Lee WK, Ko JS, Korean J. Chem. Eng., 20(3), 549 (2003)
Lee WK, McGuire J, Bothwell MK, J. Colloid Interface Sci., 252(2), 473 (2002)
Matthews BW, Remington SJ, Grtter MG, Anderson WF, J. Mol. Biol., 147, 545 (1981)

[Related Articles]

[1] Andrade JD, in Surface and Interfacial Aspects of Biomedical Polymers, J. E. Andrade Ed.,p. 1, Plenum Press, New York (1985)

[2] Proteins at interfaes II: Fundamentals and applications, Am. Cehm. Soc. Symp. Ser. Vol. 602, T. A. Horbett and J. L. Brash Eds, ACS Books, Washington DC (1995)

[3] Lundstrom I, Elwing H, J. Colloid Interface Sci., 136, 68 (1990)

[4] Kwon OH, Nho YC, Lee YM, J. Ind. Eng. Chem., 9(2), 138 (2003)

[5] Altankov G, Groth T, J. Mater. Sci. -Mater. Med., 5, 732 (1994)

[6] Thomas CH, McFarland CD, Jenkins ML, Rezania A, Steele JG, Healy KE, J. Biomed. Mater. Res., 37, 81 (1997)

[7] Norde W, Lyklema J, J. Colloid Interface Sci., 66, 257 (1978)

[8] van Dulm P, Norde W, J. Colloid Interface Sci., 91, 248 (1983)

[9] Brynda E, Houska M, Lednicky F, J. Colloid Interface Sci., 113, 164 (1986)

[10] Cho D, J. Ind. Eng. Chem., 6(5), 325 (2000)

[11] Lee WK, McGuire J, Bothwell MK, J. Colloid Interface Sci., 252(2), 473 (2002)

[12] Langmuir I, J. Am. Chem. Soc., 40, 1361 (1918)

[13] Choi HJ, Lee H, Kim KR, Ahn DH, J. Ind. Eng. Chem., 8(6), 586 (2002)

[14] Aizenbud B, Volterra V, Priel Z, J. Colloid Interface Sci., 103, 133 (1985)

[15] Young BR, Pitt WG, Cooper SL, J. Colloid Interface Sci., 124, 28 (1988)

[16] Wojciechowski PW, Brash JL, J. Colloid Interface Sci., 140, 239 (1990)

[17] Lundstrom I, Prog. Colloid Polym. Sci., 70, 76 (1985)

[18] Krisdhasima V, McGuire J, Sproull R, J. Colloid Interface Sci., 154, 337 (1992)

[19] Djardin P, Cottin I, Colloids Surf. B: Biointerfaces, 4, 111 (1995)

[20] Matthews BW, Dahlquist FW, Maynard AY, J. Mol. Biol., 78, 575 (1973)

[21] Weaver LH, Mathews BW, J. Mol. Biol., 193, 189 (1987)

[22] Sun D, Soderlind E, Baase WA, Wozniak JA, Sauer U, Matthews BW, J. Mol. Biol., 221, 873 (1991)

[23] McGuire J, Krisdhasima V, Wahlgren MC, Arnebrant T, in Proteins at interfaces II: Fundamentals and Applications, J. Brash and T. Horbett Eds, ACS Books, Washington DC (1995)

[24] Mcguire J, Wahlgren MC, Arnebrant T, J. Colloid Interface Sci., 170(1), 182 (1995)

[25] Mcguire J, Wahlgren MC, Arnebrant T, J. Colloid Interface Sci., 170(1), 193 (1995)

[26] Cuypers PA, Corsel JW, Janssen MP, Kop JMM, Hermens WT, Hemker HC, J. Biol. Chem., 258, 2426 (1983)

[27] Lee WK, Ko JS, Korean J. Chem. Eng., 20(3), 549 (2003)

[28] Lee WK, McGuire J, Bothwell MK, J. Colloid Interface Sci., 252(2), 473 (2002)

[29] Matthews BW, Remington SJ, Grtter MG, Anderson WF, J. Mol. Biol., 147, 545 (1981)