화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.28, No.6, 478-486, November, 2004
Export Citation
완충 용액의 종류, pH 그리고 침지 시간에 따른 소장점막하조직 쉬트의 물 흡수 효과
Effect of Types of Buffer Solution, pH and Soaking Time on the Water Uptake of Small Intestinal Submucosa Sheets
김선화, 신혜원, 장지욱1, 김문석1, 조선행1, 이해방1, 강길선†
  • 전북대학교 유기신물질공학과
  • 1한국화학연구원 나노생체재료연구팀
Seon Hwa Kim, Hye Won Shin, Ji Wook Jang1, Moon Suk Kim1, Sun Hang Cho1, Hai Bang Lee1, and Gilson Khang†
  • Department of Advanced Organic Materials Engineering, Chonbuk National University, 664-14 Dukjin, Jeonju 561-756, Korea
  • 1Nano-biomaterials Laboratory, Korea Research Institute of Chemical Technology, P. O. Box 107, Yuseong, Daejon 305-600, Korea
E-mail:
초록
소장점막하조직은 콜라젠과 글리코스아미노글리칸 및 세포활성을 촉진하는 성장인자들로 구성되어 있다. 최근에는 이종이식의 면역 거부반응이 없는 생체물질로서 응용되고 있고 SIS에 함유되어 있는 성장인자는 전층 피부손상층을 치료하는데 중요한 역할을 한다고 알려져 있다. 우리는 본래의 SIS 쉬트와 아세트산으로 처리하여 팽윤시킨 SIS 쉬트를 제조하였고, 각각에 대해 1겹과 5겹의 SIS 쉬트를 제조하였다. 이를 SEM을 통해 표면 및 단면의 형태를 확인하였고, 증류수, 인산염 완충액, HBSS (Hank's balanced salt solution)완충액, 트리스 완충액, HEPES (N-[2-hydroxyethyl] piperazine-N'-[2-ethanesulfonic acid]) 완충액에서의 물 흡수성, pH에 따른 물 흡수성 그리고 시간에 따른 물 흡수성을 비교 실험하였다. 본래의 SIS 쉬트보다 산 처리된 SIS 쉬트가, 증류수보다는 완충액에서의 물 흡수성이 높음을 확인하였다. 중성 용액보다 산성과 염기성 용액에서 SIS 쉬트는 팽윤되어 더 많은 물을 흡수하였다. 또한, 증류수와 HEPES 완충액에서 시간에 따른 물 흡수성 실험을 한 결과 1일 이후부터 10일 동안 약 200% 물을 지속적으로 흡수하였다. 이로써 SIS 쉬트와 산 처리된 SIS 쉬트가 창상 치료를 위한 드레싱제와 생분해성이식 재료로서 사용가능하리라 판단된다.
Small intestinal submucosa (SIS) is consisted with collagen and glycosaminoglycan as well as some growth factors which can stimulate cell activity. Recently, it has been recognized that SIS has been successfully examined in the bio-medical application as biomaterials without xenograft immuno-rejection response. We prepared native SIS sheets and acid treated SIS sheets by acetic acid with 1 or 5-layered sheets, respectively. The water uptake ability of native and acid treated SIS sheets was examined to evaluate the possibility as wound dressings. Morphologies of SIS sheets were characterized by SEM and the effects of various buffer solutions and different pH solutions on the water uptake ability were observed for 16 days. We observed that the acid treated SIS sheets had higher water uptake ability than native SIS sheets. Also, the water uptake ability of these was slightly higher in various buffers than distilled water. In conclusion, this study suggests that native and acid treated SIS sheets could be useful for the applications of wound dressing and biodegradable injectable materials.
Keywords:small intestinal submucosa;sheet;swelling;water uptake;wound dressing
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