To analyze the impact of gravity on the self-organization of nanospheres on substrates, drops of an aqueous suspension containing 60-nm polystyrene nanospheres were evaporated on different biomaterials. By reducing the speed of evaporation and by selecting different substrate positions, the interplay between gravitational force and pattern formation could be studied in detail. Results reveal limits for the gravitational force: for rings formed by nanosuspension droplets with diameters below I mm, the effect of normal gravitational force can be neglected.