분자 각인된 고분자를 이용한 카페인 흡착평형식의 결정

홍승표; 최두영; 노경호; Seung Pyo Hong; Du Youn Choi; Kyung Ho Row

Journal of the Korean Industrial and Engineering Chemistry, Vol.15, No.4, 478-481, June, 2004

Export Citation

분자 각인된 고분자를 이용한 카페인 흡착평형식의 결정

Determination of Adsorption Isotherm of Caffeine By Using Molecular Imprinted Polymer

홍승표, 최두영, 노경호 ^†

초정밀생물분리기술센터 인하대학교 화학공학과, 인천 402-751

Seung Pyo Hong, Du Youn Choi, and Kyung Ho Row^†

Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 253, Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea

E-mail:

초록

분자각인은 기능성 단량체와 과량의 가교제와의 공중합이 이루어진 고분자내에 특이적인 결합 자리를 만드는 기술이다. 본 연구에서는 카페인의 흡착평형식을 구하기 위해 제조된 카페인 각인 고분자를 사용하여 정적방법으로 실험을 하였다. 고정상과 이동상의 평형데이타는 Freundlich, Langmuir, SIPS, Radke-Prausnitz 흡착평형식들에 적용시켰다. 비선형 회귀분석에 의하여 실험변수들을 구하여 흡착평형식들 간의 일치되는 정도를 비교하였다.

The technique of molecular imprinting consists of self-assembly of a functional monomer and a template molecule in solution, followed by co-polymerization of the functional monomer in excess of an appropriate cross-linking monomer. In this work, a static method was experimentally implemented to measure adsorption isotherm of caffeine by the prepared molecular imprinting polymer. The equilibrium data of stationary and mobile phase were correlated to the Freundlich, Langmuir, SIPS and Radke-Prausnitz isotherms, respectively. By a nonlinear regression analysis, the experimental parameters in the equilibrium isotherms were estimated and compared.

Keywords:caffeine;molecular imprinted polymer;static method;adsorption isotherm

[References]

Cheong SH, Oh CY, Seo JI, Par JK, Korean J. Biotechnol. Bioeng., 16, 115 (2001)
Cheong SH, Suh MG, Park JK, Karube I, HWAHAK KONGHAK, 36(1), 27 (1998)
Stephan EM, Wong OS, Stobaugh JF, J. Pharm. Biomedical Anal., 27, 719 (2002)
Xie J, Zhu L, Luo H, Zhou L, Li C, Xu X, J. Chromatogr. A, 934, 1 (2001)
Theodoridis G, Manesiotis P, J. Chromatogr. A, 948, 163 (2002)
Matsui J, Nicholls IA, Takeuchi T, Anal. Chim. Acta, 365, 89 (1998)
Andrea B, Tolokan A, Horvai G, Horvath V, Lanza F, Hall AJ, Sellergren B, J. Chromatogr. A, 930, 31 (2001)
Masque N, Marce M, Borrull F, TrAC Trends Anal. Chem., 20, 477 (2001)
Ansell RJ, Kriz D, Mosbach K, Curr. Opin. Biotechnol., 7, 89 (1996)
Mathewkrotz J, Shea KJ, J. Am. Chem. Soc., 118(34), 8154 (1996)
Hong JM, Andersson PE, Qian J, Martin CR, Chem. Mater., 10, 1029 (1998)
Yoshikawa M, Izumi J, Kitao T, Chem. Lett., 8, 611 (1996)
Yoshikawa M, Izumi J, Kitao T, Sakamoto S, Macromolecules, 29(25), 8197 (1996)
Yoshikawa M, Izumi J, Kitao T, Polym. J., 29, 205 (1997)
Guiochon G, Golshan-Shirazi S, Katti A, Fundamentals of Preparative and Nonlinear Chromatography, Academic Press, New York (1994)
Barford RA, HPLC in Biotechnology, W.S. Hancock, Ed., Academic Press, New York (1992)
Choi YS, Row KH, J. Korean Ind. Eng. Chem., 11(3), 344 (2000)

[Related Articles]

[1] Cheong SH, Oh CY, Seo JI, Par JK, Korean J. Biotechnol. Bioeng., 16, 115 (2001)

[2] Cheong SH, Suh MG, Park JK, Karube I, HWAHAK KONGHAK, 36(1), 27 (1998)

[3] Stephan EM, Wong OS, Stobaugh JF, J. Pharm. Biomedical Anal., 27, 719 (2002)

[4] Xie J, Zhu L, Luo H, Zhou L, Li C, Xu X, J. Chromatogr. A, 934, 1 (2001)

[5] Theodoridis G, Manesiotis P, J. Chromatogr. A, 948, 163 (2002)

[6] Matsui J, Nicholls IA, Takeuchi T, Anal. Chim. Acta, 365, 89 (1998)

[7] Andrea B, Tolokan A, Horvai G, Horvath V, Lanza F, Hall AJ, Sellergren B, J. Chromatogr. A, 930, 31 (2001)

[8] Masque N, Marce M, Borrull F, TrAC Trends Anal. Chem., 20, 477 (2001)

[9] Ansell RJ, Kriz D, Mosbach K, Curr. Opin. Biotechnol., 7, 89 (1996)

[10] Mathewkrotz J, Shea KJ, J. Am. Chem. Soc., 118(34), 8154 (1996)

[11] Hong JM, Andersson PE, Qian J, Martin CR, Chem. Mater., 10, 1029 (1998)

[12] Yoshikawa M, Izumi J, Kitao T, Chem. Lett., 8, 611 (1996)

[13] Yoshikawa M, Izumi J, Kitao T, Sakamoto S, Macromolecules, 29(25), 8197 (1996)

[14] Yoshikawa M, Izumi J, Kitao T, Polym. J., 29, 205 (1997)

[15] Guiochon G, Golshan-Shirazi S, Katti A, Fundamentals of Preparative and Nonlinear Chromatography, Academic Press, New York (1994)

[16] Barford RA, HPLC in Biotechnology, W.S. Hancock, Ed., Academic Press, New York (1992)

[17] Choi YS, Row KH, J. Korean Ind. Eng. Chem., 11(3), 344 (2000)