The biological process for phosphorus removal from wastewater is based on the use of bacteria capable of accumulating inorganic polyphosphate (polyP). We previously showed that a phoU mutation leads to polyP accumulation in Escherichia coli. The phoU mutant could be easily screened on agar plates containing 5-bromo-4-chloro-3-indolyl-phosphate (X-P-i) after N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis. Here, we demonstrate that this method is also useful for screening polyP-accumulating mutants of bacterial strains isolated from soil and activated sludge samples.