Plasma Protein Adsorption to Anion Substituted Poly(vinyl alcohol) Membranes

Kyu Eun Ryu; Hyangshuk Rhim; Chong Won Park; Heung Jae Chun; Seung Hwa Hong; Jae Jin Kim; Young Moo Lee

Macromolecular Research, Vol.11, No.6, 451-457, December, 2003

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Plasma Protein Adsorption to Anion Substituted Poly(vinyl alcohol) Membranes

Kyu Eun Ryu, Hyangshuk Rhim, Chong Won Park¹, Heung Jae Chun^†, Seung Hwa Hong², Jae Jin Kim³, and Young Moo Lee⁴

Department of Biomedical Sciences, Catholic University, Seoul 137-701, Korea
¹Division of Hematology, Department of Internal Medicine,College of Medicine, Catholic University, Seoul 137-701, Korea
²Blood Products Division, Biologics Evaluation Department, Korea Food and Drug Administration, Seoul 122-704, Korea
³Biomaterials Research Center, Korea Institute of Science and Technology, Seoul 130-650, Korea
⁴School of Chemical Engineering, College of Engineering, Hanyang University, Seoul 133-791, Korea

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Anion-substituted poly(vinyl alcohol) (PVA) membranes, carboxymethylated PVA (C-PVA), and sulfonated PVA (S-PVA) were prepared and the effects of these substitutions on the plasma protein adsorption were studied by one- and two-dimensional gel electrophoresis and immunoblotting. When Cuprophane was used as a negative control, the amount of total proteins bound to samples decreased in the order Cuprophane > PVA > C-PVA > S-PVA, which we attribute to the effects of the surface characteristics of the samples, such as their surface tensions and electrostatic properties, on the adsorption of proteins to the surfaces of the materials. The results revealed that albumin was the most abundant protein in all the samples. The proportion of adsorbed fibrinogen to S-PVA exceeded those of PVA and C-PVA, whereas S-PVA exhibited the lowest IgG adsorption affinity among the samples we studied.

Keywords:carboxymethylated poly(vinyl alcohol);sulfonated poly(vinyl alcohol);hemodialysis membrane;plasma protein adsorption.

[References]

Andrade JD, Principles of Protein Adsorption in Surface and Interfacial Aspects of Biomedical Polymers, Plenum Publishing, New York, pp. 1-80 (1985)
Horbett TA, Protein Adsorption on Biomaterials in Biomaterials: Interfacial Phenomena and Applications, S.L. Cooper and N.A. Peppas, Eds., ACS Advances in Chemistry Series, ACS, Washington D.C., vol. 199, pp. 233-244 (1982)
Mulzer SR, Brash JL, J. Biomed. Mater. Res., 23, 1483 (1989)
Parzer S, Balcke P, Mannhalter C, J. Biomed. Mater. Res., 27, 455 (1993)
Chun HJ, Kim JJ, Kim KY, Polym. J., 22, 347 (1990)
Finch A, Poly(vinyl alcohol)-Development, John Wiley & Sons, Ltd., New York (1992)
Chun HJ, Kim JJ, Lee SH, Kim KY, Kim UY, Polym. J., 22, 477 (1990)
Nam SY, Chun HJ, Lee YM, J. Appl. Polym. Sci., 72(2), 241 (1999)
Khang G, Choi MK, Rhee JM, Lee SJ, Lee HB, Iwasaki Y, Nakabayashi N, Ishihara K, Korea Polym. J., 9(2), 107 (2001)
Hong KC, Kim J, Bae JY, Korea Polym. J., 9(5), 253 (2001)
Owens D, J. Appl. Polym. Sci., 13, 1711 (1969)
Min SK, Kim JH, Chung DJ, Korea Polym. J., 9(3), 143 (2001)
Berger M, Broxup B, Sefton MV, J. Mater. Sci.- Mater. Medicine, 5, 622 (1994)
Tanzi MC, Muttoni M, Fumero R, Scuri S, Cairo G, Polymers in Medicine III, C. Migliaresi, Ed., Elsevier Science Publishers B.V., Amsterdam, pp. 99-110 (1987)
Wessel D, Flugge UI, Anal. Biochem., 138, 141 (1984)
Laemmli UK, Nature, 227, 680 (1990)
Galletti PM, Colton CK, Lysaght MJ, The Biomedical Engineering Handbook, J.D. Bronzino, Ed., CRC Press, Inc., pp. 1898-1922 (1995)
Ikada Y, Iwata H, Horii F, Matsunaga T, Tanigushi M, Suzuki M, J. Biomed. Mater. Res., 15, 697 (1981)
Duncan AC, Sefton MV, Brash JL, Biomaterials, 18, 1585 (1997)
Lee JH, Kim HW, Pak PK, Kim SS, Lee HB, Korea Polym. J., 2(1), 32 (1994)
Han DK, Park KD, Ryu GH, Kim UY, Min BG, Kim YH, J. Biomed. Mater. Res., 30, 23 (1996)
Kiremitci M, Gok E, Ates S, J. Biomater. Sci.-Polym. Ed., 6, 425 (1994)
Vroman L, Bull. N.Y. Acad. Med., 64, 352 (1988)
Slack SM, Bohnert JL, Horbett TA, Ann. N.Y. Acad. Sci., 516, 223 (1987)
Gessner A, Lieske A, Paulke BR, Muller RH, Eur. J. Pharm. Biopharm., 54, 165 (2002)
Grasel TG, Cooper SL, J. Biomed. Mater. Res., 23, 311 (1989)
Santerre JP, Vanderkaup NH, Brash JL, J. Biomed. Mater. Res., 26, 39 (1992)
Breemhaar W, Brinkman E, Elleus DJ, Bengeling T, Bantjes A, Biomaterials, 5, 269 (1984)
Harfenist EJ, Packham MA, Mustard JF, Blood, 64, 1163 (1984)
Sugiyama T, Okuma M, Ushikubi F, Seusaki S, Kanaji K, Uchino H, Blood, 69, 1712 (1987)
Wettero J, Askeudal A, Bengtsson T, Tengvall P, Biomaterials, 23, 981 (2002)
Roitt I, Brostoff J, Male D, Immunology, 5th Edition, Mosby International Ltd., London, pp. 43-59 (1998)

[Referenced By]

[Related Articles]

[1] Andrade JD, Principles of Protein Adsorption in Surface and Interfacial Aspects of Biomedical Polymers, Plenum Publishing, New York, pp. 1-80 (1985)

[2] Horbett TA, Protein Adsorption on Biomaterials in Biomaterials: Interfacial Phenomena and Applications, S.L. Cooper and N.A. Peppas, Eds., ACS Advances in Chemistry Series, ACS, Washington D.C., vol. 199, pp. 233-244 (1982)

[3] Mulzer SR, Brash JL, J. Biomed. Mater. Res., 23, 1483 (1989)

[4] Parzer S, Balcke P, Mannhalter C, J. Biomed. Mater. Res., 27, 455 (1993)

[5] Chun HJ, Kim JJ, Kim KY, Polym. J., 22, 347 (1990)

[6] Finch A, Poly(vinyl alcohol)-Development, John Wiley & Sons, Ltd., New York (1992)

[7] Chun HJ, Kim JJ, Lee SH, Kim KY, Kim UY, Polym. J., 22, 477 (1990)

[8] Nam SY, Chun HJ, Lee YM, J. Appl. Polym. Sci., 72(2), 241 (1999)

[9] Khang G, Choi MK, Rhee JM, Lee SJ, Lee HB, Iwasaki Y, Nakabayashi N, Ishihara K, Korea Polym. J., 9(2), 107 (2001)

[10] Hong KC, Kim J, Bae JY, Korea Polym. J., 9(5), 253 (2001)

[11] Owens D, J. Appl. Polym. Sci., 13, 1711 (1969)

[12] Min SK, Kim JH, Chung DJ, Korea Polym. J., 9(3), 143 (2001)

[13] Berger M, Broxup B, Sefton MV, J. Mater. Sci.- Mater. Medicine, 5, 622 (1994)

[14] Tanzi MC, Muttoni M, Fumero R, Scuri S, Cairo G, Polymers in Medicine III, C. Migliaresi, Ed., Elsevier Science Publishers B.V., Amsterdam, pp. 99-110 (1987)

[15] Wessel D, Flugge UI, Anal. Biochem., 138, 141 (1984)

[16] Laemmli UK, Nature, 227, 680 (1990)

[17] Galletti PM, Colton CK, Lysaght MJ, The Biomedical Engineering Handbook, J.D. Bronzino, Ed., CRC Press, Inc., pp. 1898-1922 (1995)

[18] Ikada Y, Iwata H, Horii F, Matsunaga T, Tanigushi M, Suzuki M, J. Biomed. Mater. Res., 15, 697 (1981)

[19] Duncan AC, Sefton MV, Brash JL, Biomaterials, 18, 1585 (1997)

[20] Lee JH, Kim HW, Pak PK, Kim SS, Lee HB, Korea Polym. J., 2(1), 32 (1994)

[21] Han DK, Park KD, Ryu GH, Kim UY, Min BG, Kim YH, J. Biomed. Mater. Res., 30, 23 (1996)

[22] Kiremitci M, Gok E, Ates S, J. Biomater. Sci.-Polym. Ed., 6, 425 (1994)

[23] Vroman L, Bull. N.Y. Acad. Med., 64, 352 (1988)

[24] Slack SM, Bohnert JL, Horbett TA, Ann. N.Y. Acad. Sci., 516, 223 (1987)

[25] Gessner A, Lieske A, Paulke BR, Muller RH, Eur. J. Pharm. Biopharm., 54, 165 (2002)

[26] Grasel TG, Cooper SL, J. Biomed. Mater. Res., 23, 311 (1989)

[27] Santerre JP, Vanderkaup NH, Brash JL, J. Biomed. Mater. Res., 26, 39 (1992)

[28] Breemhaar W, Brinkman E, Elleus DJ, Bengeling T, Bantjes A, Biomaterials, 5, 269 (1984)

[29] Harfenist EJ, Packham MA, Mustard JF, Blood, 64, 1163 (1984)

[30] Sugiyama T, Okuma M, Ushikubi F, Seusaki S, Kanaji K, Uchino H, Blood, 69, 1712 (1987)

[31] Wettero J, Askeudal A, Bengtsson T, Tengvall P, Biomaterials, 23, 981 (2002)

[32] Roitt I, Brostoff J, Male D, Immunology, 5th Edition, Mosby International Ltd., London, pp. 43-59 (1998)