Derivative of chitosan was synthesized by reacting chitosan with octanoic acids. The contact angles on chitosan-octanotate film were measured using dispersion(D), polar(P) and hydrogen(H) liquids. All nonpolar liquids of alkane homologues easily spread on the surface of chitosan-octanotate film. On the other hand, polar or hydrogen-bonding liquids form a certain angle on the surface of chitosan-octanotate film. The critical surface tensions γ_c of chitosan-octanotate film were evaluated by the Zisman plot(cosθ versus γ_L) and the other plots. New functional film for MA packaging was developed to understand the relationship between surface chemistry and wettability of chitosan-octanotate film.