Removal of Aggregates of Protein from Its Self-assembled Monolayer on Metal Substrates

Soong Ho Um; Jong Bum Lee; Jeong-Woo Choi; Kee-Kahb Koo

Journal of Industrial and Engineering Chemistry, Vol.9, No.3, 243-247, May, 2003

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Removal of Aggregates of Protein from Its Self-assembled Monolayer on Metal Substrates

Soong Ho Um, Jong Bum Lee, Jeong-Woo Choi , and Kee-Kahb Koo^†

Department of Chemical Engineering, Sogang University, Seoul 121-742, Korea

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Elution ability of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) was found to segregate readily the aggregates of ferredoxin from its self-assembled monolayer onto metal substrates without a loss of the intrinsic property of ferredoxin: a high-resolution AFM image showed that the self-assembled ferredoxin monolayer on the (100) surface of the silicon substrate consisted of ferredoxin monomers or small clusters when the surface was treated with CHAPS. The electric property of the monolayer of ferredoxin on the gold surface was also confirmed to remain intact by the cyclic voltammetry measurements. Those results offer a new technique for the elimination of nonspecific adsorption of proteins on inorganic surfaces, which has been a long-term problem in the fabrication of biomolecular electronic devices.

Keywords:biomolecular electronic device;ferredoxin;self-assembled monolayer;CHAPS

[References]

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Gittins D, Bethell D, Schiffrin DJ, Nichols R, Nature, 408, 67 (2000)
Dagani R, Chem. Eng. News, 78(42), 27 (2000)
Friis EP, Andersen JET, Kharlats YI, Kuznetsov AM, Nichols RJ, Zhang JD, Ulstrup J, Proc. Natl. Acad. Sci. U.S.A., 96, 1379 (1999)
Joachim C, Gimzewski JK, Aviram A, Nature, 408, 541 (2000)
Klein DL, Roth R, Lim AK, Alivisatos AP, Mceuen PL, Nature, 389(6652), 699 (1997)
Chen J, Reed MA, Rawlett AM, Tour JM, Science, 286, 1550 (1999)
Choi JW, Nam YS, Park SJ, Lee WH, Kim DH, Fujihira M, Biosensors Bioelectronics, 16, 819 (2001)
Choi JW, Park SJ, Nam YS, Lee WH, Fujihira M, Colloids Surf. B: Biointerfaces, 23, 295 (2002)
Choi JW, Nam Ys, Kong BS, Choi HG, Lee WH, Fujihira M, Colloids Surf. B: Biointerfaces, 23, 263 (2002)
Baszkin A, Norde W, Physical Chemistry of Biological Interfaces, p. 49, Marcel Dekker, New York (2000)
Bollag D, Rozycki MD, Eedelstein SJ, Protein Methods, p. 1, Willey-Liss, New York (1996)
Harder P, Grunze M, Dahint R, Whitesides GM, Laibinis PE, J. Phys. Chem. B, 102(2), 426 (1998)
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Green RJ, Su TJ, Lu JR, Webster JRP, J. Phys. Chem. B, 105(38), 9331 (2001)
Wahlgren M, Arnebrant T, Colloids Surf. B: Biointerfaces, 6, 63 (1996)
Deutscher MP, Methods in Enzymology (182) Guide to Protein Purification, p. 239, Academic Press, San Diago (1990)
Crispin DJ, Street G, Valey JE, Food Chem., 72, 355 (2001)
Kimitto A, Holzenburg A, Ford RC, J. Biol. Chem., 272(31), 19497 (1997)
Um SH, Lee JB, Choi JW, Koo KK, Colloids Surf. B: Biointerfaces, submitted (2003)
Demers LM, Ginger DS, Park SJ, Li Z, Chung SM, Mirkin CA, Science, 296, 1836 (2002)
Christopher MAB, Maria OB, Electrochemistry: Principle, Methods, and Applications, p. 367, Oxford University Press, Oxford (1994)

[Referenced By]

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[1] Bachtold A, Hadley P, Nakanishi T, Dekker C, Science, 294, 1317 (2001)

[2] Gittins D, Bethell D, Schiffrin DJ, Nichols R, Nature, 408, 67 (2000)

[3] Dagani R, Chem. Eng. News, 78(42), 27 (2000)

[4] Friis EP, Andersen JET, Kharlats YI, Kuznetsov AM, Nichols RJ, Zhang JD, Ulstrup J, Proc. Natl. Acad. Sci. U.S.A., 96, 1379 (1999)

[5] Joachim C, Gimzewski JK, Aviram A, Nature, 408, 541 (2000)

[6] Klein DL, Roth R, Lim AK, Alivisatos AP, Mceuen PL, Nature, 389(6652), 699 (1997)

[7] Chen J, Reed MA, Rawlett AM, Tour JM, Science, 286, 1550 (1999)

[8] Choi JW, Nam YS, Park SJ, Lee WH, Kim DH, Fujihira M, Biosensors Bioelectronics, 16, 819 (2001)

[9] Choi JW, Park SJ, Nam YS, Lee WH, Fujihira M, Colloids Surf. B: Biointerfaces, 23, 295 (2002)

[10] Choi JW, Nam Ys, Kong BS, Choi HG, Lee WH, Fujihira M, Colloids Surf. B: Biointerfaces, 23, 263 (2002)

[11] Baszkin A, Norde W, Physical Chemistry of Biological Interfaces, p. 49, Marcel Dekker, New York (2000)

[12] Bollag D, Rozycki MD, Eedelstein SJ, Protein Methods, p. 1, Willey-Liss, New York (1996)

[13] Harder P, Grunze M, Dahint R, Whitesides GM, Laibinis PE, J. Phys. Chem. B, 102(2), 426 (1998)

[14] Sigal GB, Mrksich M, Whitesides GM, J. Am. Chem. Soc., 120(14), 3464 (1998)

[15] Green RJ, Su TJ, Lu JR, Webster JRP, J. Phys. Chem. B, 105(38), 9331 (2001)

[16] Wahlgren M, Arnebrant T, Colloids Surf. B: Biointerfaces, 6, 63 (1996)

[17] Deutscher MP, Methods in Enzymology (182) Guide to Protein Purification, p. 239, Academic Press, San Diago (1990)

[18] Crispin DJ, Street G, Valey JE, Food Chem., 72, 355 (2001)

[19] Kimitto A, Holzenburg A, Ford RC, J. Biol. Chem., 272(31), 19497 (1997)

[20] Um SH, Lee JB, Choi JW, Koo KK, Colloids Surf. B: Biointerfaces, submitted (2003)

[21] Demers LM, Ginger DS, Park SJ, Li Z, Chung SM, Mirkin CA, Science, 296, 1836 (2002)

[22] Christopher MAB, Maria OB, Electrochemistry: Principle, Methods, and Applications, p. 367, Oxford University Press, Oxford (1994)