We prepared a capsule containing Saccharomyces cerevisiae cells for the removal of lead(II) ion. Saccharomyces cerevisiae cells were encapsulated and cultured in the growth medium for 36 hrs. The cells grew in the capsule during cultivation and did not leak through the capsule membrane. The dried cell density reached 250 g/l on the basis of the inner volume of the capsule. The capsule was cross-linked using triethylene tetramine and glutaric dialdehyde solutions. The optimum pH of the lead uptake using encapsulated S. cerevisiae was found to be 6. The Freundlich model showed a little better fit to adsorption data than the Langmuir model. The lead uptake of the encapsulated S. cerevisiae was about 30 mg Pb/g biomass. The 95 percent of leads adsorbed on the encapsulated biosorbents was desorbed by the 1M HCI solution. The capsule was reused 50 batches without losing the metal uptake capacity. And the mechanical strength of the cross-linked capsule was retained after 50 trials.