A TRF(Tritium Removal Facility) will be constructed at Wolsong Plant to remove the tritium from heavy water. Titanium sponge has been selected as suitable material for tritium product immobilization. This study explains the experimental results conducted for the various properties of reaction between titanium sponge and hydrogen isotopes. The activation temperature required for hydriding of titanium was determined 500 ℃. The hydriding reaction between hydrogen and titanium was very fast with rapid increasing of temperature and reached to the saturated state within 10 minutes. There was no significant isotopic effect in the reaction of hydrogen and deuterium and the hydriding rates were prevented by helium content in reacting hydrogen gas.