Neocarzinostatin (NCS), one of the outstanding 9 membered cyclic enediyne antitumor natural products, is produced by S. carzinostaticus ATCC15944 and comprised of 1:1 complex of non-peptide chromophore and peptide apoperotein (NcsA). All the bioactivities are restored into the chromophore, which consists of molecularly diverse epoxybicyclo [7.3.0] dodecadienediyne core and naphthalene moiety. The aromatic moiety is glycosylated by an unusual sugar (methylated fucosamine). All three distinct parts are in protection of apoprotein. By adopting a strategy for the cloning the biosynthetic gene clusters by dTDP 4,6 dehydratase and apoprotein as probes, we have localized 130-kb showed similarity with 6-methylasalicylic acid synthase and orsellinic acid synthase. The heterologus expression of this gene into S. lividans TK24 showed as an important PKS type I gene involved in the aromatic Naphtholic moiety biosynthesis.