High cell density cultivation of glutathione-producing recombinant E. coli TG1/pDG7α was carried out in a fed-batch mode controlling specific growth rate. The cell growth and the production of GSH-I were not affected by the initial glucose concentration less than 20g/L. The specific growth rate sharply increased for the yeast extract concentration less than 1g/L. The cell growth rate and the GSH-I production rate were lowered significantly at the initial concentration of acetic acid above 5g/L. The cell growth and the GSH-I production were inhibited by acetic acid. The final cell concentration reached its maximum at 147(OD⁶⁰⁰) with μ=0.3hr^-1. The final concentration of GSH-I reached 134 unit/L with μ=0.1hr^-1. When the specific growth rate was controlled at 0.4hr^-1, the final cell concentration decreased drastically due to the accumulation of acetic acid in the medium, which resulted in the decrease of the concentration of GSH-I.