Journal of Bioscience and Bioengineering, Vol.94, No.4, 368-370, 2002
Rapid multiplex single nucleotide polymorphism genotyping based on single base extension reactions and color-coded beads
A Single nucleotide polymorphism (SNP) typing method using color-coded beads is promising because it is easy to use and inexpensive. However, the present protocols are not suitable for clinical and diagnostic applications because they need centrifugation for bead-washing. Here, we developed a simplified protocol without a bead-washing procedure that enables SNP typing of PCR amplified fragments in only 30 min.