In this work we reported the observations, using scanning tunneling microscopy (STM), on the solvent effects upon the expression of supramolecular chirality on the graphite surface in the two-dimensional assembly of 3,5-di(4-n-tetradecyloxyphenyl) pyrazole (DTPP). It was observed that DTPP dissolved in a 1:3 solvent mixture of toluene and chloroform can form chiral lamellae, while no chiral characteristics can be found when DTPP is dissolved in toluene alone. Rod-shaped molecular cores are observed in the assembly from toluene solution, while bent-shaped molecules are observed in the solvent mixture of toluene and chloroform. The observation suggests that surface-stabilized supramolecular chiral lamellae may be appreciably affected by solvents through tuning of intermolecular hydrogen-bond configuration.