Effects of temperature on hydrogen sulfide removal efficiency has been studied in this research. Two reactors which combined iron oxidization reactor and reduction reactor were manufactured. Iron oxidation reactor was used to oxidize Fe(II) to Fe(III) with Thiobacillus ferrooxidans and the hydrogen sulfide was oxidized to make element sulfur with the reduction of iron. The optimal temperature for the iron oxidation was 30 ℃ however that of iron reduction was 40 ℃. From this result, the optimal continuous operation conditions has been determined.