Non-viral gene carriers continue to attract a great deal of interest due to advantageous safety profile. Among the non-viral gene carriers, cationic liposomes or synthetic gene carriers are efficient DNA carriers in vitro, but there in vivo applications are greatly hampered because of their low bioavailability. On the other hand, chitosan, a natural cationic polysaccharide, is a candidate non-viral vector for gene delivery because of its high positive charges and low cytotoxicity. In this work, AWBP-PEG-LMWSC was synthesized by conjugation the arterial wall binding peptide (AWBP), a specific targeting peptide, to the end of PEGylation low molecular water soluble chitosan (LMWSC) as a gene carrier to target artery wall cells. Chatacters of the synthesized AWBP-PEG-LMWSC were analyzed by measuring FT-IR, ¹H-NMR, zeta-potentiometer, atomic force microscopy (AFM), transmittance electro microscopy (TEM). And the physical and biochemical properties of AWBP-PEG-LMWSC/pDNA complexes will be also investigated.