Biotechnology Letters, Vol.24, No.23, 2005-2009, 2002
One-step purification of xylanase from Melanocarpus albomyces and ethylene glycol as a novel soluble additive for enhancing its thermal stability
Hydrophobic interaction chromatography with Phenyl Sepharose 6 Fast Flow resulted in 7-fold purification of xylanase activity from Melanocarpus albomyces with over 60% recovery of the activity. The purified preparation consisted of two major isoenzymes out of seven present in the organism. Ethylene glycol, used as the eluent, enhanced the thermal stability of the xylanase activity from a half-life of 2.2 h at 55 degreesC to almost 40 h.