반응표면분석법을 이용한 알파셀룰로오스 가수분해 반응조건 최적화

오경근; 김승욱; 정용섭; 홍석인

HWAHAK KONGHAK, Vol.34, No.4, 418-423, August, 1996

Export Citation

반응표면분석법을 이용한 알파셀룰로오스 가수분해 반응조건 최적화

Optimiaztion of Opera- tion Conditions for the Hydrolysis of Cellulose by Response Surface Methodology

오경근, 김승욱, 정용섭, 홍석인

초록

환원당의 농도를 극대화시키기 위하여, 반응표면분석법을 이용하여 섬유소 가수분해 반응조건을 최적화 시키고자 하였다. 효소역가, 기질농도, 그리고 반응온도를 독립변수로 선정하였으며, 종속변수로는 환원당 농도와 생산수율(단위효소당 환원당 농도)을 정하고, 분석하였다. 3변수, 3수준, 분류인자 실험계획법에 따라 5가지 스타포인트와 4번의 중심점 반복실험을 포함하여, 모두 17가지 조건에서 실험을 수행하였다. 본 실험에서 얻은 섬유소 가수분해의 최적조건은 효소역가가 274 FPU, 기질농도가 8.9%, 그리고 반응온도는 51℃이었으며, 이 조건에서 최대 환원당 농도, 47.89 g/L를 얻었다.

To obtain the maximize the concentration of reducing sugar, optimum operating conditions for the hydrolysis of cellolose was investigated by using the response surface methodology. The independent variables were enzyme activity, substrate concentration and reaction temperature and reducing sugar concentration and production yield(reducing sugar concentration per amount of enzyme) were dependent variables. Following the 3³-fractional factorial experimental design, 17 experiments were carried out at various conditions, with 5 starpoints and 4 replicates at the center point. The optimum condition for the hydrolysis of cellulose was as follows, enzyme activity; 274 FPU, substrate concentration; 8.9%, and reaction temperature; 51℃. The maximum production of reducing sugar, 47.89 g/L, was ob- tained in this optimum condition.

Keywords:Jydrolysis of Cellulose;Optimization;Response Surface Methodology

[References]

Nowell GP, Energy Policy, Sep., 652 (1990)
Ghose TK, Roychoury PK, Ghosh P, Biotechnol. Bioeng., 26, 377 (1984)
Takagi M, Abe S, Suzuki S, Emert GH, Yata N, Proceed. Bioconversion Symp., IIT, 551 (1977)
Wright JD, Chem. Eng. Prog., 62 (1988)
Okazaki M, Moo-Young M, Biotechnol. Bioeng., 20, 637 (1978)
Holtzapple MT, Caram HS, Humphery AE, Biotechnol. Bioeng., 26, 775 (1984)
Philippidis GP, Smith TK, Wyman CE, Biotechnol. Bioeng., 41, 846 (1993)
Spindler DD, Wyman CE, Grohmann K, Biotechnol. Bioeng., 34, 189 (1989)
Ghose TK, Pure Appl. Chem., 59(2), 257 (1987)
Lee JM, "Biochemical Engineering," Prentice Hall, New Jersey, p. 94 (1992)
Miller GL, Anal. Chem., 31, 426 (1959)
Bergmeyer HU, Bernt E, Methods Enzymatic Anal., 13, 1205 (1974)
Huang SY, Chen JCJ, Ferment. Technol., 66(5), 509 (1988)
Oh KK, Jung HW, Park JY, Lim JK, Kim JC, Korean J. Food Sci. Technol., 25(1), 1 (1993)
Sattler W, Esterbauer H, Glatter O, Steiner W, Biotechnol. Bioeng., 33, 1221 (1989)
Kautola H, Gronlund B, Horitsr H, Linko YY, Linko P, Proc. 4th European Congress on Biotechnology, 1, 106 (1987)
Kautola H, Vassilev N, Linko YY, J. Biotechnol., 13, 315 (1990)
Box GEP, Hunter G, Hunter JS, "Statistics for Experimenters," John Wiley & Sons, N.Y., p. 510 (1978)
Kautola H, Vassilev N, Linko YY, Biotechnol. Lett., 11(5), 313 (1989)

[Referenced By]

[1] Nowell GP, Energy Policy, Sep., 652 (1990)

[2] Ghose TK, Roychoury PK, Ghosh P, Biotechnol. Bioeng., 26, 377 (1984)

[3] Takagi M, Abe S, Suzuki S, Emert GH, Yata N, Proceed. Bioconversion Symp., IIT, 551 (1977)

[4] Wright JD, Chem. Eng. Prog., 62 (1988)

[5] Okazaki M, Moo-Young M, Biotechnol. Bioeng., 20, 637 (1978)

[6] Holtzapple MT, Caram HS, Humphery AE, Biotechnol. Bioeng., 26, 775 (1984)

[7] Philippidis GP, Smith TK, Wyman CE, Biotechnol. Bioeng., 41, 846 (1993)

[8] Spindler DD, Wyman CE, Grohmann K, Biotechnol. Bioeng., 34, 189 (1989)

[9] Ghose TK, Pure Appl. Chem., 59(2), 257 (1987)

[10] Lee JM, "Biochemical Engineering," Prentice Hall, New Jersey, p. 94 (1992)

[11] Miller GL, Anal. Chem., 31, 426 (1959)

[12] Bergmeyer HU, Bernt E, Methods Enzymatic Anal., 13, 1205 (1974)

[13] Huang SY, Chen JCJ, Ferment. Technol., 66(5), 509 (1988)

[14] Oh KK, Jung HW, Park JY, Lim JK, Kim JC, Korean J. Food Sci. Technol., 25(1), 1 (1993)

[15] Sattler W, Esterbauer H, Glatter O, Steiner W, Biotechnol. Bioeng., 33, 1221 (1989)

[16] Kautola H, Gronlund B, Horitsr H, Linko YY, Linko P, Proc. 4th European Congress on Biotechnology, 1, 106 (1987)

[17] Kautola H, Vassilev N, Linko YY, J. Biotechnol., 13, 315 (1990)

[18] Box GEP, Hunter G, Hunter JS, "Statistics for Experimenters," John Wiley & Sons, N.Y., p. 510 (1978)

[19] Kautola H, Vassilev N, Linko YY, Biotechnol. Lett., 11(5), 313 (1989)