A detailed chemical kinetic mechanism has been developed and used to study the oxidation of iso-octane in a jet-stirred reactor, flow reactors, shock tube,, and in a motored engine. Over the series of experiments investigated, the initial pressure ranged from 1 to 45 atm. the temperature from 550 K to 1700 K. the equivalence ratio from 0.3 to 1.5. with nitrogen-argon dilution from 70% to 99%. This range of physical conditions, together with the measurements of ignition delay time and concentrations, provide a broad-ranging test of the chemical kinetic mechanism. This mechanism was based on our precious modeling of alkane combustion and, in particular, on our study of the oxidation of n-heptane. Experimental results of ignition behind reflected shock waves were used to develop and validate the predictive capability of the reaction mechanism at both low and high temperatures. Moreover, species' concentrations from flow reactors and a jet-stirred reactor Acre used to help complement and refine the low and intermediate temperature portions of the reaction mechanism, leading to good predictions of intermediate products in most cases. In addition. a sensitivity analysis was performed for each of the combustion environments in an attempt to identify the most important reactions under the relevant conditions of study.