화학공학소재연구정보센터
Biotechnology Letters, Vol.24, No.15, 1249-1252, 2002
Removal of carboxymethyl cellulase activity from the culture filtrate of Termitomyces clypeatus producing xylanase
Termitomyces clypeatus produced 450 IU xylanase ml(-1) in a medium containing starch-free wheat bran powder as the carbon source. Carboxymethyl cellulase (CMCase) activity in the culture filtrate was removed by keeping the filtrate at pH 10 for 60 min followed by a change to pH 6. Treatment of Kraft pulp (bamboo) with the filtrate at pH 7 decreased the kappa number from 10.5 to 5 with release of reducing groups equivalent to 0.15 mg glucose g(-1) pulp.