Study on the synthesis of chemical nucleases that have ability of cleavage of RNA or DNA to investigate the structure of nucleic acids was performed. We synthesized Ni-complex that was synthesized by Karn and Busch and identified the specificity and ability as chemical nuclease by Chen et. al., and Cu-complex and Cd-complex that were presumed as having specificity similar to Ni-complex in terms of the structure and specificity and ability as chemical nuclease. The spectra of synthesized new transition metal complexes were compared with those of Ni-complex. Results of comparing data of UV and IR spectra of Cu-complex and Cd-complex with those of Ni-complex showed that the structures of newly synthesized transition metal complexes were similar to the structure of Ni-complex. The yield of synthesis of Ni-complex, Cu-complex, and Cd-complex was 81%, 7%, and 39% respectively.