Three-layer core-shell latex particles with fluid cores were used for the preparation of a polymeric nanostructured material. The fluid cores were synthesized from the low glass transition temperature core-forming polymer (CFP) covalently labeled with a fluorescent dye. The CFP was encapsulated with a thin rigid shell of shell-forming polymer 1 (SFP-1). The second shell was synthesized from the shell-forming polymer 2 (SFP-2) on the surface of the bilayer particles. The specific relationship between the glass transition temperatures, T-g's, of the polymers incorporated into the core-shell particles was T-g(CFP) < T-g(SFP-2) < T-g(SFP-1). Nanocomposite polymeric films were obtained following heat processing of the arrays of core-shell particles at annealing temperature, T-g(SFP-2) < T-ann < T-g(SFP-1). The morphology of the nanostructured material was studied by examining the distribution of the fluorescent dye in the polymer film. Various factors providing sharp modulation in optical properties of the composite films were examined and discussed.