Biotechnology Letters, Vol.24, No.6, 445-448, 2002
Repeated elicitation enhances taxane production in suspension cultures of Taxus chinensis in bioreactors
Methyl jasmonate added at 100 muM on days 8, 14 and 20 to cultures of Taxus chinensis growing in flasks and 1 l airlift bioreactors improved taxuyunnanine C (TC) production by 50-60% in flasks and by about 80% in airlift bioreactors compared with single addition of 100 muM methyl jasmonate on day 8. The final TC production in the airlift bioreactors reached 565+/-47 mg l(-1), which is the highest reported for taxoid production in a bioreactor.
Keywords:elicitation;physiologically active substance;plant cell culture;taxoid production;Taxus chinensis