Journal of Bioscience and Bioengineering, Vol.92, No.4, 393-396, 2001
Construction of a combinatorial protein library displayed on yeast cell surface using DNA random priming method
The random DNA fragments, generated from mRNAs of Saccharomyces cerevisiae using a novel "DNA random priming method", were inserted into a surface display vector to construct a combinatorial random protein library. Its surface display was confirmed by immunofluorescence labeling of the RGS (His)(6) reporter inserted, and its potential was also evaluated.
Keywords:combinatorial random protein library;DNA random priming;yeast cell surface engineering;surface display