Journal of Bioscience and Bioengineering, Vol.90, No.3, 332-334, 2000
Efficient preparation of optically active ketoprofen by Mucor javanicus lipase immobilized on an inorganic support
Lipase M from Mucor javanicus, one of nine commercially available hydrolytic enzymes, showed good enantioselectivity (E=50) for racemic ketoprofen trifluoroethyl ester in phosphate buffer (pH 7.0) containing 30% acetone. Lipase M immobilized on Toyonite 200-A showed the best selectivity (E=55) and reactivity. Moreover, the lipase could be recycled at least 5 times.