Journal of Bioscience and Bioengineering, Vol.87, No.1, 93-96, 1999
Identification of an insertion sequence, IS1203 variant, in a Shiga toxin 2 gene of Escherichia coli O157 : H7
An insertion sequence composed of 1310 bp was found in Shiga toxin 2 genes of some isolates of Escherichia coil O157:H7. This insertion sequence showed extremely high homology with IS1203 of E. coil O111:H-. This IS1203 variant was inserted in the region encoding the amino-terminus of the B subunit with a duplication of 3 bp at the target site, resulting in inactivation of the Shiga toxin 2 gene.