Journal of Chemical Technology and Biotechnology, Vol.77, No.2, 137-140, 2002
Biotransformation of benzaldehyde to L-phenylacetylcarbinol (L-PAC) by Torulaspora delbrueckii and conversion to ephedrine by microwave radiation
In a 5 dM(3) stirred tank reactor, bioconversion of 30g benzaldehyde by cells of Torulaspora delbrueckii yielded 22.9g of pure L-phenylacetylcarbinol (L-PAC). Facile functional group transform, formation of 4.5g of L-PAC to 2-(methylimino)-1-phenyl-1-propanol by exposure to microwave irradiation for 9min resulted in 2.48g of product. Conversion of 4.8g of 2-(methylimino)-1-phenyl-1-propanol to 3.11g of ephedrine was achieved by exposure to microwaves in a reaction time of 10min. The identity of all the products was confirmed by H-1 NMR and FT-IR analysis.
Keywords:benzaldehyde;biotransformation;ephedrine;L-phenylacetylcarbinol;imine formation;microwave irradiation;reduction;Torulaspora delbrueckii