포도당 산화효소반응을 이용한 3상 실관 생화학반응기의 해석

장호남; 최상교; 김경원; 이창우

HWAHAK KONGHAK, Vol.31, No.5, 547-555, October, 1993

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포도당 산화효소반응을 이용한 3상 실관 생화학반응기의 해석

Analysis of Three-Phase Hollow Fiber Bioreactor Using Enzymatic Glucose Oxidation

장호남, 최상교, 김경원, 이창우

초록

실관의 다공성 부분에 포도당 산화효소를 삽입하고 그 위에 실리콘 코팅을 하여 산소를 통하게 한 모형 삼상실관반응기의 거동에 관한 이론과 실험을 행하였다. 포도당의 농도가 75mM 이상일 때는 산소가 율속단계가 되는 것을 이론과 실험을 통하여 확인하였다. 실험을 통한 유효인자는 예측치 보다 15-25%의 오차속에 잘 일치하였다.

Experimental and theoretical studies on three-phase hollow fiber bioreactor were performed using an asymmetric hollow fiber in which glucose oxidase was entrapped in the porous sponge region and silicone coating was applied on top of that. Oxygen limitation has been confirmed by theory and experiment when the glucose concentration was above 75mM. The measured effectiveness factor was lower by 15-25% than the predicted one, which may be attributed to the deactivation of the enzyme upon immobilization. The theoretical and experimental values were in good agreement within error limits of 15-25%.

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[1] Rony PR, Biotechnol. Bioeng., 13, 341 (1971)

[2] Knazek RA, Guillino PM, Kohler PO, Dedrick RL, Science, 178, 65 (1972)

[3] Kim DJ, Chang HN, Liu JR, Biotechnol. Tech., 3, 139 (1989)

[4] Chang HN, "Advances in Biochemical Engineering/Biotechnology," 44, Springer-Verlag, Berlin, 27 (1991)

[5] Robertson CR, Kim IH, Biotechnol. Bioeng., 27, 1012 (1985)

[6] Chung BH, Chang HN, Biotechnol. Bioeng., 32, 205 (1988)

[7] Chang HN, Kyung YS, Chung BH, Biotechnol. Bioeng., 29, 552 (1987)

[8] Steinmyer DE, Shuler ML, Biotechnol. Prog., 6, 286 (1990)

[9] Sim SJ, Chang HN, Korean J. Biotechnol. Bioeng., in press (1992)

[10] Horvath C, Shendalman LH, Light RT, Chem. Eng. Sci., 28, 375 (1973)

[11] Waterland LR, Michaels AS, Robertson CR, AIChE J., 20, 50 (1974)

[12] Lewis W, Middleman S, AIChE J., 20, 1012 (1974)

[13] Georgakis C, Chan PCH, Aris R, Biotechnol. Bioeng., 17, 99 (1975)

[14] Kim SS, Cooney DO, Chem. Eng. Sci., 31, 289 (1976)

[15] Webster IA, Shuler ML, Biotechnol. Bioeng., 21, 1725 (1979)

[16] Webster IA, Shuler ML, Chem. Eng. Sci., 34, 1273 (1979)

[17] Webster IA, Shuler ML, Biotechnol. Bioeng., 23, 447 (1981)

[18] Chang HN, Chung BH, Korean J. Chem. Eng., 5, 83 (1987)

[19] Rovito BJ, Kittrell JR, Biotechnol. Bioeng., 15, 143 (1973)

[20] Gibson QH, Swoboda BEP, Massey V, J. Biol. Chem., 239, 3927 (1964)

[21] Nakamura S, Ogura Y, Biochemistry, 63, 308 (1968)

[22] Bright HJ, Gibson QH, J. Biol. Chem., 242, 994 (1967)

[23] Duke FR, Weibel MK, Page DS, Bulgrin VG, Luthy J, J. Am. Chem. Soc., 91, 3904 (1969)

[24] Bright HJ, Appleby M, J. Biol. Chem., 244, 3625 (1969)

[25] Weibel MK, Bright HJ, J. Biol. Chem., 246, 2734 (1971)

[26] Chang HN, AIChE J., 28, 1031 (1982)

[27] Fink DF, Na TY, Schultz JS, Biotechnol. Bioeng., 15, 879 (1973)

[28] Bischoff KB, AIChE J., 11, 351 (1965)

[29] Waterland LR, Robertson CR, Michaels AS, Chem. Eng. Commun., 2, 37 (1975)

[30] Cho YK, Bailey JE, Biotechnol. Bioeng., 25, 1923 (1983)

[31] Clark DS, Bailey JE, Biotechnol. Bioeng., 24, 1027 (1982)

[32] Krerelen DW, "Properties of Polymers: Their Estimation and Correlation with Chemical Structure," 2nd ed., Elsevier Scientific, Amsterdam, pp. 403-425 (1976)

[33] Reuss M, Buchholz K, Biotechnol. Bioeng., 21, 2061 (1979)

[34] Duke FR, Weibel MK, Page DS, Bulgrin VG, Luthy J, J. Am. Chem. Soc., 91, 3901 (1969)