Bioresource Technology, Vol.82, No.1, 61-64, 2002
Pigeon pea waste as a novel, inexpensive, substrate for production of a thermostable alkaline protease from thermoalkalophilic Bacillus sp JB-99
Thermoalkaliphilic Bacillus sp. JB-99 was grown in a 250 nil Erlenmeyer flask containing 50 nil medium containing (g/1) Pigeon pea waste 10; NaNO3, 5.0; K2HPO4, 5.0; MgSO4 . 2H(2)O, 0.2 and Na2CO3, 10.0. Incubations were carried out at 50 degreesC on a rotary incubator shaker for 15 h. A high level of extra cellular thermostable protease activity was observed after 24 h incubation. The optimum temperature and pH for activity were 70 degreesC and 11, respectively, so this enzyme showed stable activity at high temperature and under alkaline conditions.