Human fibroblast growth factor-2 can be used to induce angiogenesis in ischemias and wound healing. Site-directed mutagenesis of bovine FGF-2 cDNA was performed in order to produce the human-form of FGF-2 in E. coli. The mitogenic, angiogenic and neurotrophic activities of the recovered protein were analysed by [H-3]thymidine uptake to DNA of cultured fibroblasts, rabbit ear dermal ulcers wound healing and neuronal differentiation of PC12 cells.