The sorption behavior of chlorobenzene on marine sediments treated by different methods was investigated. Through experiments on three factors (temperature, sediment organic carbon content, and media) that might affect the sorption behavior of chlorobenzene, some important conclusions were obtained. Three hours were required for adsorption equilibrium of chlorobenzene on marine sediments. Adsorption of chlorobenzene was completed mainly via the surface and microporosity action of sediment. Either the Freundlich model or the Langmuir model was fit for describing adsorption behavior of chlorobenzene on marine sediments. Temperature did not have a great influence on adsorption behavior of chlorobenzene in seawater, but the saturate sorption amount decreased as the temperature became high. Through study on adsorption of chlorobenzene in different media, we found that the saturate sorption amount of chlorobenzene in natural seawater was several times greater than that in artificial seawater or deionized water.