Fluoropolymer films prepared by radio frequency glow discharge (RF-glow discharge) are of interest as biomaterials coatings. Previous studies focused on hexafluoroethane (C2F6, HFE) and tetrafluoroethylene (C2F4, TFE) as the monomer precursor have shown such surfaces to exhibit unique protein binding capabilities. In this study, slow, surface directed deposition of hexafluoropropylene (C3F6, HFP) films is shown to confer surface functional group presentation that promotes high protein retention. The surface chemistry is controllable over a range of values, and C3F6 films prepared by RF-glow discharge are smooth, homogeneous, and defect free. Surface patterns of the fluoropolymer can be created by direct or photolithographic techniques. Scanning probe microscopic analysis indicates a surface modulus in the range of 1.2 < E-HFP < 5.5 GPa. These results are discussed in terms of the applicability of C3F6 films for coating biomaterials.